4.5 Article

Methylome-wide analysis of IVF neonates that underwent embryo culture in different media revealed no significant differences

Journal

NPJ GENOMIC MEDICINE
Volume 7, Issue 1, Pages -

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s41525-022-00310-3

Keywords

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Funding

  1. March of Dimes [6-FY13-153]
  2. stichting fertility foundation
  3. EVA (Erfelijkheid Voortplanting & Aanleg) speciality programme of Maastricht University Medical Centre (MUMC+) [KP111513]
  4. Horizon 2020 innovation (ERIN) of the European Commission [EU952516]
  5. Environment, Nature and Energy Department of the Flemish government
  6. Flemish Government (Department of Economy, Science and Innovations)
  7. Flemish Government (Agency for Care and Health)
  8. Flemish Government (Department of Environment)

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There were no significant methylation differences in the umbilical cord blood methylome between IVF neonates developed in different IVF culture media, suggesting that the use of different culture media may not affect the epigenetic profile of IVF newborns.
A growing number of children born are conceived through in vitro fertilisation (IVF), which has been linked to an increased risk of adverse perinatal outcomes, as well as altered growth profiles and cardiometabolic differences in the resultant individuals. Some of these outcomes have also been shown to be influenced by the use of different IVF culture media and this effect is hypothesised to be mediated epigenetically, e.g. through the methylome. As such, we profiled the umbilical cord blood methylome of IVF neonates that underwent preimplantation embryo development in two different IVF culture media (G5 or HTF), using the Infinium Human Methylation EPIC BeadChip. We found no significant methylation differences between the two groups in terms of: (i) systematic differences at CpG sites or regions, (ii) imprinted sites/genes or birth weight-associated sites, (iii) stochastic differences presenting as DNA methylation outliers or differentially variable sites, and (iv) epigenetic gestational age acceleration.

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