4.6 Article

An Orthotopic Patient-Derived Xenograft (PDX) Model Allows the Analysis of Metastasis-Associated Features in Colorectal Cancer

Journal

FRONTIERS IN ONCOLOGY
Volume 12, Issue -, Pages -

Publisher

FRONTIERS MEDIA SA
DOI: 10.3389/fonc.2022.869485

Keywords

patient-derived xenograft (PDX); colorectal cancer (CRC); metastasis; epithelial-to-mesenchymal transition (EMT); organoids

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Metastasis is the leading cause of death in colorectal cancer patients, making it imperative to develop preclinical models that can replicate the process of metastasis at the individual patient level. In this study, an orthotopic patient-derived xenograft (PDX) model was established by directly implanting freshly dissociated colorectal cancer cells into the colon of immunocompromised mice. The findings suggest that ortho-PDX can be a useful preclinical model to study metastasis-related features and demonstrate that the epithelial-to-mesenchymal transition and stemness properties of primary colorectal tumors may play a crucial role in tumor engraftment.
Metastasis is the primary cause of death in patients with colorectal cancer (CRC), urging the need for preclinical models that recapitulate the metastatic process at the individual patient level. We used an orthotopic patient-derived xenograft (PDX) obtained through the direct implantation of freshly dissociated CRC cells in the colon of immunocompromised mice to model the metastatic process. Ortho-PDX engraftment was associated to a specific set of molecular features of the parental tumor, such as epithelial-to-mesenchymal transition (EMT), TGF-beta pathway activation, increased expression of stemness-associated factors and higher numbers of circulating tumor cells (CTCs) clusters expressing the metastatic marker CD44v6. A parallel analysis of orthotopic/metastatic xenografts and organoids showed that tumor cells underwent mesenchymal-to-epithelial transition at the metastatic site and that metastasis-derived organoids had increased chemotherapy resistance. These observations support the usefulness of ortho-PDX as a preclinical model to study metastasis-related features and provide preliminary evidence that EMT/stemness properties of primary colorectal tumors may be crucial for orthotopic tumor engraftment.

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