Journal
CELLS
Volume 11, Issue 15, Pages -Publisher
MDPI
DOI: 10.3390/cells11152269
Keywords
human renal cystic tissue; ADPKD; chorioallantoic membrane (CAM) model; mouse kidney slices; 3D in vivo model; polycystic kidney disease
Categories
Funding
- Deutsche Forschungsgemeinschaft (DFG) [HA 6655/1-3, AU 635/1-1, BU 2918/3-1]
- DFG [387509280, SFB 1350]
Ask authors/readers for more resources
Autosomal dominant polycystic kidney disease (ADPKD) is a genetic disorder that leads to the progressive enlargement of kidney cysts and renal failure. Current models for studying human cyst growth and drug trials are limited. In this study, a chorioallantoic membrane (CAM) model was used to culture renal tissue from ADPKD patients and mouse kidney slices, and successfully evaluated cystic tissue growth. The CAM model may provide a valuable platform for bridging the gap between animal studies and clinical trials of human cyst growth.
(1) Background: Autosomal dominant polycystic kidney disease (ADPKD) is a frequent monogenic disorder that leads to progressive renal cyst growth and renal failure. Strategies to inhibit cyst growth in non-human cyst models have often failed in clinical trials. There is a significant need for models that enable studies of human cyst growth and drug trials. (2) Methods: Renal tissue from ADPKD patients who received a nephrectomy as well as adult mouse kidney slices were cultured on a chorioallantoic membrane (CAM) for one week. The cyst volume was monitored by microscopic and CT-based applications. The weight and angiogenesis were quantified. Morphometric and histological analyses were performed after the removal of the tissues from the CAM. (3) Results: The mouse and human renal tissue mostly remained vital for about one week on the CAM. The growth of cystic tissue was evaluated using microscopic and CT-based volume measurements, which correlated with weight and an increase in angiogenesis, and was accompanied by cyst cell proliferation. (4) Conclusions: The CAM model might bridge the gap between animal studies and clinical trials of human cyst growth, and provide a drug-testing platform for the inhibition of cyst enlargement. Real-time analyses of mouse kidney tissue may provide insights into renal physiology and reduce the need for animal experiments.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available