An acoustofluidic scanning nanoscope using enhanced image stacking and processing

Nanoscale optical resolution with a large field of view is a critical feature for many research and industry areas, such as semiconductor fabrication, biomedical imaging, and nanoscale material identification. Several scanning microscopes have been developed to resolve the inverse relationship between the resolution and field of view; however, those scanning microscopes still rely upon fluorescence labeling and complex optical systems. To overcome these limitations, we developed a dual-camera acoustofluidic nanoscope with a seamless image merging algorithm (alpha-blending process). This design allows us to precisely image both the sample and the microspheres simultaneously and accurately track the particle path and location. Therefore, the number of images required to capture the entire field of view (200 x 200 mu m) by using our acoustofluidic scanning nanoscope is reduced by 55-fold compared with previous designs. Moreover, the image quality is also greatly improved by applying an alpha-blending imaging technique, which is critical for accurately depicting and identifying nanoscale objects or processes. This dual-camera acoustofluidic nanoscope paves the way for enhanced nanoimaging with high resolution and a large field of view.

Automated summary

Combining nanoscale optical resolution with a large field of view is crucial in many research and industry areas. To overcome limitations of existing scanning microscopes, a dual-camera acoustofluidic nanoscope has been developed, allowing for precise imaging of samples and microspheres simultaneously. This innovation reduces the number of images needed for capturing the entire field of view by 55-fold compared to previous designs, while greatly improving image quality through an alpha-blending imaging technique.