Pathological changes induced by Alzheimer's brain inoculation in amyloid-beta plaque-bearing mice

Alzheimer's disease (AD) is characterized by intracerebral accumulations of extracellular amyloid-beta (A beta) plaques and intracellular tau pathology that spread in the brain. Three types of tau lesions occur in the form of neuropil threads, neurofibrillary tangles, and neuritic plaques i.e. tau aggregates within neurites surrounding A beta deposits. The cascade of events linking these lesions and synaptic or memory impairments are still debated. Intracerebral infusion of human AD brain extracts in A beta plaque-bearing mice that do not overexpress pathological tau proteins induces tau pathologies following heterotopic seeding of mouse tau protein. There is however little information regarding the downstream events including synaptic or cognitive repercussions of tau pathology induction in these models. In the present study, human AD brain extracts (AD(be)) and control-brain extracts (Ctrl(be)) were infused into the hippocampus of A beta plaque-bearing APP(swe)/PS1(dE9) mice. Memory, synaptic density, as well as A beta plaque and tau aggregate loads, microgliosis, astrogliosis at the inoculation site and in connected regions (perirhinal/entorhinal cortex) were evaluated 4 and 8 months post-inoculation. AD(be) inoculation produced the following effects: (i) memory deficit; (ii) increased A beta plaque deposition in proximity to the inoculation site; (iii) tau pathology induction; (iv) appearance of neuropil threads and neurofibrillary tangles next to the inoculation site with a spreading to connected regions. Neuritic plaque pathology was detected in both AD(be)- and Ctrl(be)-inoculated animals but AD(be) inoculation increased the severity close to and at distance of the inoculation site. (v) Finally, AD(be) inoculation reduced synaptic density in the vicinity to the inoculation site and in connected regions as the perirhinal/entorhinal cortex. Synaptic impairments were correlated with increased severity of neuritic plaques but not to other tau lesions or A beta lesions, suggesting that neuritic plaques are a culprit for synaptic loss. Synaptic density was also associated with microglial load.

Automated summary

Alzheimer's disease (AD) is characterized by the accumulation of amyloid-beta (Aβ) plaques and tau pathology in the brain. In this study, human AD brain extracts were injected into mice, leading to tau pathology, memory deficits, and reduced synaptic density. Neuritic plaques were found to be responsible for synaptic loss.