Journal
JOURNAL OF ENVIRONMENTAL RADIOACTIVITY
Volume 162, Issue -, Pages 182-188Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.jenvrad.2016.05.034
Keywords
Persimmon tannin; Ultrasound-assisted extraction; Purification; Anti-radiation
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Funding
- National Nature Science Foundation of China [21265003, 81460451]
- Fund of Guangxi Key Laboratory of Metabolic Diseases Research [2015-181h-02]
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In this study, persimmon tannin was extracted from Diospyros kaki L.f. using ultrasound-assisted extraction and purified by D101 macroporous resin column chromatography and polysulfone ultrafiltration membrane. The tannin content of the final persimmon tannin extracts was attained to 39.56% calculated as catechin equivalents. Also, the radioprotective effects of persimmon tannin for HEK 293T cells proliferation and apoptosis after Gamma irradiation were investigated by CCK-8, Hoechst 33258 staining, flow cytometry assay and intracellular reactive oxygen species assay (ROS). Persimmon tannin was pre-incubated with HEK 293T cells for 12 h prior to Gamma irradiation. It was found that pretreatment with persimmon tannin increased cell viability and inhibited generation of Gamma-radiation induced ROS in HEK 293T cells exposed to 8 Gy Gamma-radiation. The percentage of apoptotic cells were only 6.7% when the radiation dose was 8 Gy and pretreated with 200 mu g/ml of persimmon tannin. All these results indicated that persimmon tannin offered a potent radioprotective effect on cell vitality and cell apoptosis of Gamma-radiation exposure in HEK 293T cells. This study would serve as a pre-clinical evaluation of persimmon tannin for use in people with radiation protection: (C) 2016 Elsevier Ltd. All rights reserved.
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