4.3 Article

Dysbiotic human oral microbiota alters systemic metabolism via modulation of gut microbiota in germ-free mice

Journal

JOURNAL OF ORAL MICROBIOLOGY
Volume 14, Issue 1, Pages -

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1080/20002297.2022.2110194

Keywords

Oral; gut; microbiome; liver; transcriptome; metabolome

Categories

Funding

  1. KAKENHI [21K21035, JP18H04067, JP16H05207]
  2. Sunstar Inc. [JP18gm0710009]
  3. JSPS
  4. Japan Agency for Medical Research and Development-Core Research for Evolutional Science and Technology (Japan Agency for Medical Research and Development (AMED)) [JP18gm0710009]

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This study demonstrates that periodontitis-associated oral microbiota can induce disease phenotype when colonizing the gut of germ-free mice, affecting gut microbial composition, hepatic gene expression profiles, and serum metabolites.
Background The effect of oral microbiota on the intestinal microbiota has garnered growing attention as a mechanism linking periodontal diseases to systemic diseases. However, the salivary microbiota is diverse and comprises numerous bacteria with a largely similar composition in healthy individuals and periodontitis patients. Aim We explored how health-associated and periodontitis-associated salivary microbiota differently colonized the intestine and their subsequent systemic effects. Methods The salivary microbiota was collected from a healthy individual and a periodontitis patient and gavaged into C57BL/6NJcl[GF] mice. Gut microbial communities, hepatic gene expression profiles, and serum metabolites were analyzed. Results The gut microbial composition was significantly different between periodontitis-associated microbiota-administered (PAO) and health-associated oral microbiota-administered (HAO) mice. The hepatic gene expression profile demonstrated a distinct pattern between the two groups, with higher expression of lipid and glucose metabolism-related genes. Disease-associated metabolites such as 2-hydroxyisobutyric acid and hydroxybenzoic acid were elevated in PAO mice. These metabolites were significantly correlated with characteristic gut microbial taxa in PAO mice. Conversely, health-associated oral microbiota were associated with higher levels of beneficial serum metabolites in HAO mice. Conclusion The multi-omics approach used in this study revealed that periodontitis-associated oral microbiota is associated with the induction of disease phenotype when they colonized the gut of germ-free mice.

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