Combatting the effect of image reconstruction settings on lymphoma [18F]FDG PET metabolic tumor volume assessment using various segmentation methods

Background [F-18]FDG PET-based metabolic tumor volume (MTV) is a promising prognostic marker for lymphoma patients. The aim of this study is to assess the sensitivity of several MTV segmentation methods to variations in image reconstruction methods and the ability of ComBat to improve MTV reproducibility. Methods Fifty-six lesions were segmented from baseline [F-18]FDG PET scans of 19 lymphoma patients. For each scan, EARL1 and EARL2 standards and locally clinically preferred reconstruction protocols were applied. Lesions were delineated using 9 semiautomatic segmentation methods: fixed threshold based on standardized uptake value (SUV), (SUV = 4, SUV = 2.5), relative threshold (41% of SUVmax [41M], 50% of SUVpeak [A50P]), majority vote-based methods that select voxels detected by at least 2 (MV2) and 3 (MV3) out of the latter 4 methods, Nestle thresholding, and methods that identify the optimal method based on SUVmax (L2A, L2B). MTVs from EARL2 and locally clinically preferred reconstructions were compared to those from EARL1. Finally, different versions of ComBat were explored to harmonize the data. Results MTVs from the SUV4.0 method were least sensitive to the use of different reconstructions (MTV ratio: median = 1.01, interquartile range = [0.96-1.10]). After ComBat harmonization, an improved agreement of MTVs among different reconstructions was found for most segmentation methods. The regular implementation of ComBat ('Regular ComBat') using non-transformed distributions resulted in less accurate and precise MTV alignments than a version using log-transformed datasets ('Log-transformed ComBat'). Conclusion MTV depends on both segmentation method and reconstruction methods. ComBat reduces reconstruction dependent MTV variability, especially when log-transformation is used to account for the non-normal distribution of MTVs.

Automated summary

This study assessed the sensitivity of different image reconstruction methods on various metabolic tumor volume (MTV) segmentation methods and the ability of ComBat to improve the reproducibility of MTVs. The results showed that the SUV4.0 method was the least sensitive to different reconstructions, and after using ComBat for harmonization, most segmentation methods showed improved agreement in MTVs among different reconstructions. The study also found that the version of ComBat using log-transformed datasets was more accurate and precise compared to the version using non-transformed distributions.