Characterization of symbiotic and nitrogen fixing bacteria

Symbiotic nitrogen fixing bacteria comprise of diverse species associated with the root nodules of leguminous plants. Using an appropriate taxonomic method to confirm the identity of superior and elite strains to fix nitrogen in legume crops can improve sustainable global food and nutrition security. The current review describes taxonomic methods preferred and commonly used to characterize symbiotic bacteria in the rhizosphere. Peer reviewed, published and unpublished articles on techniques used for detection, classification and identification of symbiotic bacteria were evaluated by exploring their advantages and limitations. The findings showed that phenotypic and cultural techniques are still affordable and remain the primary basis of species classification despite their challenges. Development of new, robust and informative taxonomic techniques has really improved characterization and identification of symbiotic bacteria and discovery of novel and new species that are effective in biological nitrogen fixation (BNF) in diverse conditions and environments.

Automated summary

This review discusses the preferred taxonomic methods for characterizing symbiotic bacteria in the rhizosphere, highlighting the continued use of phenotypic and cultural techniques despite challenges. The development of new taxonomic techniques has greatly improved the identification of symbiotic bacteria and led to the discovery of novel species effective in biological nitrogen fixation.