4.4 Article

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Journal

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 183, Pages -

Publisher

JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/62803

Keywords

-

Funding

  1. RSF [17-15-01290]
  2. RFBR [19-015-00273]
  3. Russian Science Foundation [17-15-01290] Funding Source: Russian Science Foundation

Ask authors/readers for more resources

This protocol combines EdU labeling, Ag-amplification of Nanogold particles, and ChromEM staining for visualizing replicative domains in cell culture, allowing for high-efficiency pre-embedding labeling and providing a new method for studying chromatin structure.
Principles of DNA folding in the cell nucleus and its dynamic transformations that occur during the fulfillment of basic genetic functions (transcription, replication, segregation, etc.) remain poorly understood, partially due to the lack of experimental approaches to high-resolution visualization of specific chromatin loci in structurally preserved nuclei. Here we present a protocol for the visualization of replicative domains in monolayer cell culture in situ, by combining EdU labeling of newly synthesized DNA with subsequent label detection with Ag-amplification of Nanogold particles and ChromEM staining of chromatin. This protocol allows for the high-contrast, high-efficiency pre-embedding labeling, compatible with traditional glutaraldehyde fixation that provides the best structural preservation of chromatin for room-temperature sample processing. Another advantage of pre-embedding labeling is the possibility to pre-select cells of interest for sectioning. This is especially important for the analysis of heterogeneous cell populations, as well as compatibility with electron tomography approaches to highresolution 3D analysis of chromatin organization at sites of replication, and the analysis of post-replicative chromatin rearrangement and sister chromatid segregation in the interphase.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.4
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available