Phylogenetically Distant BABY BOOM Genes From Setaria italica Induce Parthenogenesis in Rice

The combination of apomixis and hybrid production is hailed as the holy grail of agriculture for the ability of apomixis to fix heterosis of F-1 hybrids in succeeding generations, thereby eliminating the need for repeated crosses to produce F-1 hybrids. Apomixis, asexual reproduction through seed, achieves this feat by circumventing two processes that are fundamental to sexual reproduction (meiosis and fertilization) and replacing them with apomeiosis and parthenogenesis, resulting in seeds that are clonal to the maternal parent. Parthenogenesis, embryo development without fertilization, has been genetically engineered in rice, maize, and pearl millet using PsASGF?BABY BOOM-like (PsASGR-BBML) transgenes and in rice using the OsBABY BOOM1 (OsBBM1) cDNA sequence when expressed under the control of egg cell-specific promoters. A phylogenetic analysis revealed that BABY BOOM (BBM)/BBML genes from monocots cluster within three different clades. The BBM/BBML genes shown to induce parthenogenesis cluster within Glade 1 (the ASGR-BBML Glade) along with orthologs from other monocot species, such as Setaria italica. For this study, we tested the parthenogenetic potential of three BBM transgenes from S. italica, each a member of a different phylogenetic BBM Glade. All transgenes were genomic constructs under the control of the AtDD45 egg cell-specific promoter. All SiBBM transgenes induced various levels of parthenogenetic embryo development, resulting in viable haploid T-1 seedlings. Poor seed set and lower haploid seed production were characteristics of multiple transgenic lines. The results presented in this study illustrate that further functional characterization of BBMs in zygote/embryo development is warranted.

Automated summary

The combination of apomixis and hybrid production is considered the holy grail of agriculture, providing a way to fix the heterosis of F-1 hybrids in future generations. Genetically engineered parthenogenesis has been achieved in rice, maize, and pearl millet, bypassing the processes of meiosis and fertilization. In this study, the parthenogenetic potential of BBM transgenes from Setaria italica was tested, showing varying levels of embryo development.