Maize and peanut intercropping improves the nitrogen accumulation and yield per plant of maize by promoting the secretion of flavonoids and abundance of Bradyrhizobium in rhizosphere

Belowground interactions mediated by root exudates are critical for the productivity and efficiency of intercropping systems. Herein, we investigated the process of microbial community assembly in maize, peanuts, and shared rhizosphere soil as well as their regulatory mechanisms on root exudates under different planting patterns by combining metabolomic and metagenomic analyses. The results showed that the yield of intercropped maize increased significantly by 21.05% (2020) and 52.81% (2021), while the yield of intercropped peanut significantly decreased by 39.51% (2020) and 32.58% (2021). The nitrogen accumulation was significantly higher in the roots of the intercropped maize than in those of sole maize at 120 days after sowing, it increased by 129.16% (2020) and 151.93% (2021), respectively. The stems and leaves of intercropped peanut significantly decreased by 5.13 and 22.23% (2020) and 14.45 and 24.54% (2021), respectively. The root interaction had a significant effect on the content of ammonium nitrogen (NH4+-N) as well as the activities of urease (UE), nitrate reductase (NR), protease (Pro), and dehydrogenase (DHO) in the rhizosphere soil. A combined network analysis showed that the content of NH4+-N as well as the enzyme activities of UE, NR and Pro increased in the rhizosphere soil, resulting in cyanidin 3-sambubioside 5-glucoside and cyanidin 3-O-(6-Op-coumaroyl) glucoside-5-O-glucoside; shisonin were significantly up-regulated in the shared soil of intercropped maize and peanut, reshaped the bacterial community composition, and increased the relative abundance of Bradyrhizobium. These results indicate that interspecific root interactions improved the soil microenvironment, regulated the absorption and utilization of nitrogen nutrients, and provided a theoretical basis for high yield and sustainable development in the intercropping of maize and peanut.

Automated summary

Belowground interactions mediated by root exudates play a critical role in the productivity and efficiency of intercropping systems. This study investigated the assembly of microbial communities in maize, peanuts, and shared rhizosphere soil, as well as their regulatory mechanisms on root exudates under different planting patterns. The results showed that intercropped maize had significantly increased yield, while intercropped peanuts had significantly decreased yield. Root interactions had a significant effect on the content of ammonium nitrogen and the activities of related enzymes in the rhizosphere soil. Network analysis revealed a reshaped bacterial community composition and increased relative abundance of Bradyrhizobium in the shared soil of intercropped maize and peanuts. These findings suggest that interspecific root interactions improve the soil microenvironment, regulate nitrogen absorption and utilization, and provide a theoretical basis for high yield and sustainable development in maize and peanut intercropping.