Journal
FRONTIERS IN MICROBIOLOGY
Volume 13, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2022.937192
Keywords
hemagglutinin stalk; extracellular matrix protein; ebola glycoprotein GP; universal influenza A vaccine; DC targeting
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This study reveals the efficacy of a novel influenza vaccine candidate based on Ebola glycoprotein dendritic cell-targeting domain. The vaccine candidate efficiently targets immune cells and induces high titers of antibodies, providing protection against different influenza strains.
A universal influenza vaccine is required for broad protection against influenza infection. Here, we revealed the efficacy of novel influenza vaccine candidates based on Ebola glycoprotein dendritic cell (DC)-targeting domain (E Delta M) fusion protein technology. The four copies of ectodomain matrix protein of influenza (tM2e) or M2e hemagglutinin stalk (HA stalk) peptides (HM2e) were fused with E Delta M to generate E Delta M-tM2e or E Delta M-HM2e, respectively. We demonstrated that E Delta M-HM2e- or E Delta M-tM2e-pseudotyped viral particles can efficiently target DC/macrophages in vitro and induced significantly high titers of anti-HA and/or anti-M2e antibodies in mice. Significantly, the recombinant vesicular stomatitis virus (rVSV)-E Delta M-tM2e and rVSV-E Delta M-HM2e vaccines mediated rapid and potent induction of M2 or/and HA antibodies in mice sera and mucosa. Importantly, vaccination of rVSV-E Delta M-tM2e or rVSV-E Delta M-HM2e protected mice from influenza H1N1 and H3N2 challenges. Taken together, our study suggests that rVSV-E Delta M-tM2e and rVSV-E Delta M-HM2e are promising candidates that may lead to the development of a universal vaccine against different influenza strains.
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