4.7 Article

Optimized production of a bioactive human recombinant protein from the microalgae Chlamydomonas reinhardtii grown at high density in a fed-batch bioreactor

Related references

Note: Only part of the references are listed.
Article Biochemical Research Methods

Novel cis-regulatory elements as synthetic promoters to drive recombinant protein expression from the Chlamydomonas reinhardtii nuclear genome

Josie L. McQuillan et al.

Summary: In this study, novel genetic devices for transgene expression in Chlamydomonas reinhardtii have been developed by identifying cis-regulatory DNA elements capable of driving high transgene expression. This provides a new toolbox of cis-regulatory elements for driving transgene expression at various levels and reveals insights into promoter structure and gene regulation in C. reinhardtii.

NEW BIOTECHNOLOGY (2022)

Article Geochemistry & Geophysics

Highly siderophile elements in shergottite sulfides and the sulfur content of the martian mantle

Marine Paquet et al.

Summary: The composition and abundance data of sulfides in Shergottite meteorites were studied, showing that sulfide grains in depleted and intermediate Shergottites have high HSE abundances, while sulfide grains in enriched Shergottites contain elements such as gold, palladium, and rhodium. The new findings suggest that the martian mantle and its partial melts are likely similar to or slightly higher in sulfur content compared to the Earth's mantle and its partial melts.

GEOCHIMICA ET COSMOCHIMICA ACTA (2021)

Review Biochemistry & Molecular Biology

Nuclear transformation of Chlamydomonas reinhardtii: A review

Meng-Ping Zhang et al.

Summary: Chlamydomonas reinhardtii is a model organism with three sequenced genomes capable of genetic transformation, offering advantages of low cost, non-toxicity, and a posttranslational modification system ensuring recombinant proteins have the same activity as natural proteins. This review summarizes methods for nuclear transformation, genes for selection, foreign protein expression examples, and factors affecting transformation efficiency, providing insights for effective strategies.

BIOCHIMIE (2021)

Article Biotechnology & Applied Microbiology

Ultrahigh-cell-density heterotrophic cultivation of the unicellular green alga Chlorella sorokiniana for biomass production

Hu Jin et al.

Summary: A successful and scalable heterotrophic cultivation technology for Chlorella sorokiniana GT-1 has been developed, achieving ultrahigh biomass concentrations and reducing production costs. The low-starch mutant SLM2 showed lower biomass concentrations, highlighting the importance of C. sorokiniana GT-1 cells' ability to accumulate starch under heterotrophic conditions. The breakthrough in cultivation technology holds promise for the Chlorella industry, expanding its applications in food and feed.

BIOTECHNOLOGY AND BIOENGINEERING (2021)

Review Environmental Sciences

Bioplastic Production from Microalgae: A Review

Senem Onen Cinar et al.

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH (2020)

Article Biotechnology & Applied Microbiology

Nuclear genome shuffling significantly increases production of chloroplast-based recombinant protein in Chlamydomonas reinhardtii

Francis J. Fields et al.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2019)

Article Biotechnology & Applied Microbiology

Efficient heterotrophic cultivation of Chlamydomonas reinhardtii

Zhen Zhang et al.

JOURNAL OF APPLIED PHYCOLOGY (2019)

Article Biotechnology & Applied Microbiology

Downstream Processing of Chlamydomonas reinhardtii TN72 for Recombinant Protein Recovery

Laura Stoffels et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2019)

Article Biotechnology & Applied Microbiology

Optimising light conditions increases recombinant protein production in Chlamydomonas reinhardtii chloroplasts

Saskya E. Carrera Pacheco et al.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2018)

Article Biotechnology & Applied Microbiology

Fed-batch mixotrophic cultivation of Chlamydomonas reinhardtii for high-density cultures

Francis J. Fields et al.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2018)

Article Biotechnology & Applied Microbiology

High-yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii

Erick Miguel Ramos-Martinez et al.

PLANT BIOTECHNOLOGY JOURNAL (2017)

Article Biotechnology & Applied Microbiology

Synthetic promoters capable of driving robust nuclear gene expression in the green alga Chlamydomonas reinhardtii

Melissa A. Scranton et al.

ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS (2016)

Article Biotechnology & Applied Microbiology

Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit

Kyle J. Lauersen et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

Investigating the dynamics of recombinant protein secretion from a microalgal host

Kyle J. Lauersen et al.

JOURNAL OF BIOTECHNOLOGY (2015)

Article Multidisciplinary Sciences

Enhanced Genetic Tools for Engineering Multigene Traits into Green Algae

Beth A. Rasala et al.

PLOS ONE (2014)

Article Multidisciplinary Sciences

Production of unique immunotoxin cancer therapeutics in algal chloroplasts

Miller Tran et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Review Biochemistry & Molecular Biology

Strategies for the Production of Recombinant Protein in Escherichia coli

Gopal Jee Gopal et al.

PROTEIN JOURNAL (2013)

Review Biotechnology & Applied Microbiology

Best practices in heterotrophic high-cell-density microalgal processes: achievements, potential and possible limitations

Fabian Bumbak et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2011)

Review Biotechnology & Applied Microbiology

Micro-algae come of age as a platform for recombinant protein production

Elizabeth Specht et al.

BIOTECHNOLOGY LETTERS (2010)

Review Energy & Fuels

Biofuels from algae: challenges and potential

Michael Hannon et al.

BIOFUELS-UK (2010)

Article Biochemical Research Methods

Factors effecting expression of vaccines in microalgae

Raymond Surzycki et al.

BIOLOGICALS (2009)

Review Biotechnology & Applied Microbiology

Bioreactor scale-up and oxygen transfer rate in microbial processes: An overview

Felix Garcia-Ochoa et al.

BIOTECHNOLOGY ADVANCES (2009)

Article Plant Sciences

Strategies to facilitate transgene expression in Chlamydomonas reinhardtii

Alke Eichler-Stahlberg et al.

PLANTA (2009)

Review Biochemical Research Methods

Chlamydomonas reinhardtii chloroplasts as protein factories

Stephen P. Mayfield et al.

CURRENT OPINION IN BIOTECHNOLOGY (2007)

Review Biotechnology & Applied Microbiology

Advanced genetic strategies for recombinant protein expression in Escherichia coli

HP Sorensen et al.

JOURNAL OF BIOTECHNOLOGY (2005)

Article Biotechnology & Applied Microbiology

High-yield production of lutein by the green microalga Chlorella protothecoides in heterotrophic fed-batch culture

XM Shi et al.

BIOTECHNOLOGY PROGRESS (2002)

Review Biochemistry & Molecular Biology

Chlamydomonas as a model organism

EH Harris

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY (2001)