Journal
POLYMERS
Volume 14, Issue 13, Pages -Publisher
MDPI
DOI: 10.3390/polym14132643
Keywords
polymeric polyelectrolyte multilayers; bone mesenchymal stromal cells; QCM-D; cell spreading; cytostasis
Categories
Funding
- University of Catania
Ask authors/readers for more resources
In this study, the effects of two different endings of polyelectrolytes assembled layer-by-layer on the cell adhesion, growth, and viability of human bone mesenchymal stromal cells (MSCs) were investigated. The results showed that the substrate with polystyrene sulfonate (PSS) ending was the most suitable for promoting cell adhesion and maintaining the phenotype of MSCs, while the interaction with polyethylenimine (PEI) significantly affected cell growth and viability.
Polyelectrolytes assembled layer-by-layer (PEMs) are commonly used as functional coatings to build-up biological interfaces, particularly suitable as compatible layers for the interaction with a biological medium, providing suitable conditions to promote or prevent cell seeding while maintaining the phenotype. The proper assessment of the biocompatibility of PEMs and the elucidation of the related mechanisms are therefore of paramount importance. In this study, we report in detail the effect of two different PEM endings, polystyrene sulfonate (PSS) and polyethylenimine (PEI), respectively, on the cell adhesion, growth, and viability of human bone mesenchymal stromal cells (MSCs). The results have shown that PSS-ended substrates appear to be the most suitable to drive the cell adhesion and phenotype maintenance of MSCs, showing good biocompatibility. On the contrary, while the cells seem to adhere more quickly and strongly on the PEI-ended surfaces, the interaction with PEI significantly affects the growth and viability, reducing the cell spreading capability, by sequestering the adhesion molecules already in the very early steps of cell-substrate contact. These results point to the promotion of a cytostatic effect of PEI, rather than the often-claimed cytotoxicity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available