Journal
PLOS BIOLOGY
Volume 20, Issue 8, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pbio.3001681
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Funding
- Great Lakes Bioenergy Research Center
- U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-SC0018409]
- National Science Foundation Long-term Ecological Research Program [DEB 1832042]
- Michigan State University AgBioResearch
- U.S. Department of Energy [DE-SC0014156, DE-SC0017883]
- National Science PGRP Award [IOS1402393]
- Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]
- U.S. Department of Energy (DOE) [DE-SC0017883, DE-SC0014156] Funding Source: U.S. Department of Energy (DOE)
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Leaf fungal microbiomes can shape successional patterns through host genetics and environmental factors. The study also found that host immune genes are closely associated with the control of the entire leaf microbiome.
Leaf fungal microbiomes can be fundamental drivers of host plant success, as they contain pathogens that devastate crop plants and taxa that enhance nutrient uptake, discourage herbivory, and antagonize pathogens. We measured leaf fungal diversity with amplicon sequencing across an entire growing season in a diversity panel of switchgrass (Panicum virgatum). We also sampled a replicated subset of genotypes across 3 additional sites to compare the importance of time, space, ecology, and genetics. We found a strong successional pattern in the microbiome shaped both by host genetics and environmental factors. Further, we used genome-wide association (GWA) mapping and RNA sequencing to show that 3 cysteine-rich receptor-like kinases (crRLKs) were linked to a genetic locus associated with microbiome structure. We confirmed GWAS results in an independent set of genotypes for both the internal transcribed spacer (ITS) and large subunit (LSU) ribosomal DNA markers. Fungal pathogens were central to microbial covariance networks, and genotypes susceptible to pathogens differed in their expression of the 3 crRLKs, suggesting that host immune genes are a principal means of controlling the entire leaf microbiome.
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