Laccase-TEMPO as an Efficient System for Doxorubicin Removal from Wastewaters

A large number of drugs are used to treat different diseases, and thus to improve the quality of life for humans. These represent a real ecological threat, as they end up in soil or ground waters in amounts that can affect the environment. Among these drugs, doxorubicin is a highly cytotoxic compound used as anticancer medicine. Doxorubicin can be efficiently removed from wastewater or polluted water using a simple enzymatic (biocatalytic) system, employing the oxidoreductase enzyme laccase and a stable organic nitroxide-free radical, TEMPO. Results presented in this work (as percentage of removal) were obtained at pH 5 and 7, after 2, 4, 6, and 24 h, using different ratios between doxorubicin, laccase, and TEMPO. It was shown that longer time, as well as an increased amount of catalyst, led to a higher percentage of removal, up to 100%. The influence of all these parameters is also discussed. In this way it was shown that the laccase-TEMPO biocatalytic system is highly efficient in the removal of the anticancer drug doxorubicin from wastewaters.

Automated summary

The study demonstrated that the enzymatic system with laccase and TEMPO is highly efficient in removing the anticancer drug doxorubicin from wastewaters. Longer treatment time and increased catalyst amount resulted in higher removal percentage, up to 100%. The influence of various parameters on the removal efficiency was also discussed.