Recognition of the TDP-43 nuclear localization signal by importin α1/β

Cytoplasmic mislocalization of the TAR-DNA binding protein of 43 kDa (TDP-43) leads to large, insoluble aggregates that are a hallmark of amyotrophic lateral sclerosis and frontotemporal dementia. Here, we study how importin alpha 1/beta recognizes TDP-43 bipartite nuclear localization signal (NLS). We find that the NLS makes extensive contacts with importin alpha 1, especially at the minor NLS-binding site. NLS binding results in steric clashes with the C terminus of importin alpha 1 that disrupts the TDP-43 N-terminal domain (NTD) dimerization interface. A putative phosphorylation site in the proximity of TDP-43 R83 at the minor NLS site destabilizes binding to importins by reducing the NLS backbone dynamics. Based on these data, we explain the pathogenic role of several post-translationalmodifications and mutations in the proximity of TDP-43 minor NLS site that are linked to disease and shed light on the chaperone activity of importin alpha 1/beta.

Automated summary

This study investigates the interaction between the bipartite nuclear localization signal (NLS) of TDP-43 and importin alpha 1/beta. The study finds that the NLS has extensive contacts with importin alpha 1, particularly at a minor NLS-binding site, disrupting the N-terminal domain (NTD) dimerization interface of TDP-43. A potential phosphorylation site near TDP-43 R83 at the minor NLS site reduces binding to importins by affecting the dynamics of the NLS backbone.