Hepatitis, testicular degeneration, and ataxia in DIDO3-deficient mice with altered mRNA processing

Background mRNA processing is an essential step of gene expression; its malfunction can lead to different degrees of physiological disorder from subclinical disease to death. We previously identified Dido1 as a stemness marker and a gene involved in embryonic stem cell differentiation. DIDO3, the largest protein encoded by the Dido1 gene, is necessary for accurate mRNA splicing and correct transcription termination. The deletion of Dido1 exon16, which encodes the carboxy-terminal half of DIDO3, results in early embryonic lethality in mouse. Results We obtained mice bearing a Cre-LoxP conditional version of that deletion and studied the effects of inducing it ubiquitously in adult stages. DIDO3-deficient mice survive the deletion but suffer mild hepatitis, testicular degeneration, and progressive ataxia, in association with systemic alterations in mRNA splicing and transcriptional readthrough. Conclusions These results offer insight into the distinct vulnerabilities in mouse organs following impairment of the mRNA processing machinery, and could aid understanding of human health dependence on accurate mRNA metabolism.

Automated summary

Background mRNA processing is important for gene expression, and its dysfunction can cause physiological disorders. The gene Dido1 has been identified as a stemness marker involved in embryonic stem cell differentiation. Deletion of an exon of Dido1 in mice leads to early embryonic death, while mice lacking DIDO3 survive but develop mild hepatitis, testicular degeneration, and ataxia.