Antenna movements as a function of odorants' biological value in honeybees (Apis mellifera L.)

In honeybees, the antennae are highly mobile sensory organs that express scanning movements in various behavioral contexts and toward many stimuli, especially odorants. The rules underlying these movements are still unclear. Using a motion-capture system, we analyzed bees' antennal responses to a panel of pheromonal and other biologically relevant odorants. We observed clear differences in bees' antennal responses, with opposite movements to stimuli related to opposite contexts: slow backward movements were expressed in response to alarm pheromones, while fast forward movements were elicited by food related cues as well as brood and queen related pheromones. These responses are reproducible, as a similar pattern of odor-specific responses was observed in bees from different colonies, on different years. We then tested whether odorants' attractiveness for bees, measured using an original olfactory orientation setup, may predict antenna movements. This simple measure of odorants' valence did however not correlate with either antennal position or velocity measures, showing that more complex rules than simple hedonics underlie bees' antennal responses to odorants. Lastly, we show that newly-emerged bees express only limited antennal responses compared to older bees, suggesting that a significant part of the observed responses are acquired during bees' behavioral development.

Automated summary

Honeybees' antennae exhibit different responses to stimuli in different behavioral contexts. Slow backward movements are observed in response to alarm pheromones, while fast forward movements are elicited by food, brood, and queen related pheromones. The attractiveness of odorants does not correlate with antennal movements, indicating more complex rules underlying bees' antennal responses. A significant part of these responses is acquired during bees' behavioral development.