Journal
NUTRIENTS
Volume 14, Issue 15, Pages -Publisher
MDPI
DOI: 10.3390/nu14153041
Keywords
Roseburia intestinalis; butyrate; TLR5; Sp3; ulcerative colitis
Categories
Funding
- National Natural Science Foundation of China [NSFC 82070571]
- Clinical Technology Innovation and Cultivation Program of Army Medical University [CX2019JS222]
- Research Project Fund of Army Medical University [2018XLC2028]
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The pathogenesis of ulcerative colitis is unclear but is believed to be related to an imbalance in gut microbiota. This study found that Roseburia intestinalis may play a role in relieving colitis by upregulating Toll-like receptor 5 transcription.
The pathogenesis of ulcerative colitis (UC) is unclear, but it is generally believed to be closely related to an imbalance in gut microbiota. Roseburia intestinalis (R. intestinalis) might play a key role in suppressing intestinal inflammation, but the mechanism of its anti-inflammatory effect is unknown. In this study, we investigated the role of R. intestinalis and Toll-like receptor 5 (TLR5) in relieving mouse colitis. We found that R. intestinalis significantly upregulated the transcription of TLR5 in intestinal epithelial cells (IECs) and improved colonic inflammation in a colitis mouse model. The flagellin of R. intestinalis activated the release of anti-inflammatory factors (IL-10, TGF-beta) and reduced inflammation in IECs. Furthermore, butyrate, the main metabolic product secreted by R. intestinalis, regulated the expression of TLR5 in IECs. Our data show that butyrate increased the binding of the transcription factor Sp3 (specificity protein 3) to the TLR5 promoter regions, upregulating TLR5 transcription. This work provides new insight into the anti-inflammatory effects of R. intestinalis in colitis and a potential target for UC prevention and treatment.
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