4.7 Article

Electrochemical, spectroscopic and pharmacological approaches toward the understanding of biflorin DNA damage effects

Journal

JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 765, Issue -, Pages 168-178

Publisher

ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2015.09.040

Keywords

Biflorin; DNA interaction; DNA sensors; Electrochemistry of quinones; Cytotoxicity; Comet assay; Clastogenicity; Aneuploidogenicity

Funding

  1. FINEP
  2. CNPq [407963/2013-8]
  3. BNB/FUNDECI
  4. PRONEX/FAPEAL/CNPq
  5. PROCAD/CAPES [88881.068501/2014-01]
  6. RENORBIO
  7. FAPESPA
  8. FAPEAM
  9. FAPEAL
  10. INCT-Bioanalitica
  11. CAPES

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The present study aims to evaluate some aspects of the pharmacoelectrochemistry of biflorin, a biologically active 1,2-naphthoquinone derivative, isolated from the roots of Capraria biflora. Electrochemical experiments involving biflorin using single, double-strand DNA and isolated bases had shown interaction of this quinone with DNA. Similar results were obtained using spectrophotometry (UV-Vis experiments and fluorimetry). Binding constants DNA-biflorin were obtained, through differential pulse voltammetry and fluorimetry. Spectroscopic studies and thermodynamic data had shown that biflorin can intercalate through dsDNA by van der Waals interactions and hydrogen bonds. The effects of biflorin-dsDNA interaction were addressed through a molecular cytogenetic approach, using comet assay and chromosome aberration induction evaluation. Indeed, biflorin, compared to the negative control, presented approximately 4- and 6-fold increases in DNA damage index and 4.1 and 13 -fold enhanced damage frequencies at 40 and 80 pM, respectively. However, biflorin did not significantly induce chromosome aberrations, suggesting that this molecule does not possess clastogenic potential, but cytotoxic potential. The absence of either clastogenic or aneuploidogenic activity of the compound reinforced its safety. (C) 2015 Elsevier B.V. All rights reserved.

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