Journal
BIOMEDICAL OPTICS EXPRESS
Volume 13, Issue 9, Pages 4706-4717Publisher
Optica Publishing Group
DOI: 10.1364/BOE.454558
Keywords
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Funding
- NSF [ECCS-1607250]
- NIH [K08EY027458, R01EY029489]
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Emerging cell-based regenerative medicine and stem cell therapies have become significant in the field of medical research and clinical practice. This study presents a multimodality imaging technology that successfully tracks the 3D migration trajectories of labeled therapeutic cells in the eyes of living rabbits, providing valuable insights for the development of cell-based therapies.
Emerging cell-based regenerative medicine and stem cell therapies have drawn wide attention in medical research and clinical practice to treat tissue damage and numerous incurable diseases. In vivo observation of the distribution, migration, and development of the transplanted cells is important for both understanding the mechanism and evaluating the treatment efficacy and safety. However, tracking the 3D migration trajectories for individual therapeutic cells in clinically relevant pathological environments remains technically challenging. Using a laser photocoagulation model in living rabbit eyes, this study demonstrates a multimodality imaging technology integrating optical coherence tomography (OCT), fluorescence microscopy (FM), and lasing emission for in vivo longitudinal tracking of the 3D migration trajectories of individual human retinal pigment epithelium cells (ARPE-19) labeled with CdS nanowires. With unique lasing spectra generated from the subtle microcavity differences, the surface-modified nanowires perform as distinct spectral identifiers for labeling individual ARPE-19 cells. Meanwhile, with strong optical scattering and natural fluorescence emission, CdS nanowires also served as OCT and FM contrast agents to indicate the spatial locations of the transplanted ARPE-19 cells. A longitudinal study of tracking individual ARPE-19 cells in rabbit eyes over a duration of 28 days was accomplished. This method could potentially promote an understanding of the pharmacodynamics and pharmacokinetics of implanted cells in the development of cell-based therapies.(c) 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
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