Journal
BEILSTEIN JOURNAL OF NANOTECHNOLOGY
Volume 13, Issue -, Pages 549-559Publisher
BEILSTEIN-INSTITUT
DOI: 10.3762/bjnano.13.46
Keywords
cell imaging; fluorescence probes; glutathione; gold nanoparticles; mercury ions; rhodamine 6G derivatives
Funding
- National Natural Science Foundations of China [21665027]
- YMU-DEAKIN International Associated Laboratory on Functional Materials, Education Department of Yunnan Province [117-02001001002107]
- Science and Technology Department of Yunnan Province [202105AC160055]
- College Student Innovation and Entrepreneurship Training Project of China
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Functionalized gold nanoparticles (GNPs) have been widely used in sensing applications, especially for detecting metal ions. This article describes the use of 13 nm GNPs functionalized by glutathione (GSH) and conjugated with a rhodamine 6G derivative (Rh6G2) to detect Hg(II) in cells. The detection strategy is based on the ion-catalyzed hydrolysis of Rh6G2, resulting in a significant change in the fluorescence of GNPs-GSH-Rh6G2 from an OFF to an ON state.
The optical and biological properties of functionalized gold nanoparticles (GNPs) have been widely used in sensing applications. GNPs have a strong binding ability to thiol groups. Furthermore, thiols are used to bind functional molecules, which can then be used, for example, to detect metal ions in solution. Herein, we describe 13 nm GNPs functionalized by glutathione (GSH) and conjugated with a rhodamine 6G derivative (Rh6G2), which can be used to detect Hg(II) in cells. The detection of Hg2+ ions is based on an ion-catalyzed hydrolysis of the spirolactam ring of Rh6G2, leading to a significant change in the fluorescence of GNPs-GSH-Rh6G2 from an OFF to an ON state. This strategy is an effective tool to detect Hg2+ ions. In cytotoxicity experiments, GNPs-GSH-Rh6G2 could penetrate living cells and detect mercury ions through the fluorescent ON form.
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