Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 775, Issue -, Pages 58-63Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2016.05.028
Keywords
Electrochemical aptasensor; Dopamine; Redox cycling; Tris(2-carboxyethyl)phosphine; Gold nanoparticles
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Funding
- National Natural Science Foundation of China [21205003, 21305004]
- Joint Fund for Fostering Talents of National Natural Science Foundation of China [U1304205]
- Henan Province [U1304205]
- Program for Science and Technology Innovation Talents at the University of Henan Province [15HASTIT001]
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This paper presented an electrochemical aptasensor for dopamine detection with high sensitivity and selectivity. The electrochemical signal was amplified by electrochemical-chemical redox cycling with tris(-2carboxyethyl)phosphine (TCEP) as the reducing reagent. Specifically, dopamine captured by the aptamer-covered gold nanoparticles-modified carbon glass electrode was cycled by TCEP after its electrochemical oxidization, enabling an increase in the anodic current. Because of the high specificity and strong binding affinity of aptamer to dopamine, the sensor is fairly selective in not responding to common interferences. The results of chronoamperometry indicated that this aptasensor allowed for the detection of dopamine in a linear concentration range of 5 nM to 0.5 mu M. The detection limit was estimated to be 1.8 nM. Additionally, the aptasensor was successfully applied to determine dopamine spiked in the serum sample and gave recoveries ranging from 94.6 to 107.2%. This work would also be valuable for development of new types of electrochemical sensors for determination of other electroactive substances by marrying specific receptors and effective reducing or oxidizing reagents. (C) 2016 Elsevier B.V. All rights reserved.
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