Journal
NATURE COMMUNICATIONS
Volume 13, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-022-32085-7
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Funding
- Centre National de la Recherche Scientifique (CNRS)
- University of Toulouse-University Toulouse III Paul Sabatier
- Fondation pour la Recherche Medicale (FRM) [DEQ20160334902]
- Fondation Bettencourt Schueller
- Agence Nationale de la Recherche (ANR) [ANR-14-CE14-0024, ANR-21-CE11-0031]
- JPI-AMR program [JPIAMR_2018_P010]
- MSDAVENIR (grant FIGHT-TB)
- Labex ARCANE, CBH-EUR-GS [ANR-17-EURE-0003]
- CNRS
- IBiSA
- European Structural Funds
- Occitanie Region
- Agence Nationale de la Recherche (ANR) [ANR-21-CE11-0031, ANR-14-CE14-0024] Funding Source: Agence Nationale de la Recherche (ANR)
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Mycobacterium tuberculosis, the human pathogen of tuberculosis, requires metal exporter CtpC and chaperone-like protein PacL1 for resistance to zinc poisoning. PacL1 binds zinc ions and forms high-molecular-weight complexes with CtpC in the cytoplasmic membrane, playing a critical role in CtpC function. PacL proteins may act as scaffolds for metal efflux platforms mediating bacterial resistance to metal poisoning.
The human pathogen Mycobacterium tuberculosis requires a P-1B-ATPase metal exporter, CtpC (Rv3270), for resistance to zinc poisoning. Here, we show that zinc resistance also depends on a chaperone-like protein, PacL1 (Rv3269). PacL1 contains a transmembrane domain, a cytoplasmic region with glutamine/alanine repeats and a C-terminal metal-binding motif (MBM). PacL1 binds Zn2+, but the MBM is required only at high zinc concentrations. PacL1 co-localizes with CtpC in dynamic foci in the mycobacterial plasma membrane, and the two proteins form high molecular weight complexes. Foci formation does not require flotillin nor the PacL1 MBM. However, deletion of the PacL1 Glu/Ala repeats leads to loss of CtpC and sensitivity to zinc. Genes pacL1 and ctpC appear to be in the same operon, and homologous gene pairs are found in the genomes of other bacteria. Furthermore, PacL1 colocalizes and functions redundantly with other PacL orthologs in M. tuberculosis. Overall, our results indicate that PacL proteins may act as scaffolds that assemble P-ATPase-containing metal efflux platforms mediating bacterial resistance to metal poisoning. The human pathogen Mycobacterium tuberculosis requires a metal exporter, CtpC, for resistance to zinc poisoning. Here, the authors show that zinc resistance also depends on a chaperone-like protein that binds zinc ions, forms high-molecular-weight complexes with CtpC in the cytoplasmic membrane, and is required for CtpC function.
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