Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 94, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jff.2022.105107
Keywords
Fatty acid synthase; ?-Mangostin; Osteosarcoma; Apoptosis; Metastasis; Endoplasmic reticulum stress
Categories
Funding
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA23080601]
- Fundamental Research Funds for the Central Universities [2012315]
- Youth Innovation Promotion Association, CAS
Ask authors/readers for more resources
It was found that α-mangostin inhibits the proliferation and migration of osteosarcoma cells by blocking FASN expression, which may serve as a potential target for osteosarcoma treatment.
Fatty acid synthase (FASN) is highly expressed in multiple types of human cancers and is recognized as one of the therapeutic targets for treating cancer metastasis. alpha-Mangostin is a natural xanthone extracted from mangosteen fruit, and possesses various biological activities. In our previous studies, alpha-mangostin was found to inhibit FASN activity. The present study was designed to reveal the effects of alpha-mangostin on osteosarcoma and to reveal whether the mechanism of alpha-mangostin in anticancer activity is related to FASN inhibition. Cytotoxicity was assessed in osteosarcoma MG63, 143B and U2OS cells. Cell viability was detected by an MTT assay. The protein expression levels were detected by western blotting. Flow cytometry, Annexin V/propidium iodide dual staining and Hoechst 33258 staining were performed to assess the apoptotic effects. Wound healing and Transwell assays were used to detect the inhibitory effect of alpha-mangostin on osteosarcoma cells invasion and migration. We found that alpha-mangostin blocked FASN expression, inhibited osteosarcoma cell proliferation, invasion and migration in a dose-dependent manner. In addition, alpha-mangostin regulated endoplasmic reticulum transmembrane receptors and signal protein expression in osteosarcoma cells. These findings suggested that alpha-mangostin suppresses osteosarcoma cell proliferation and metastasis by inhibiting FASN expression, which provides a basis as a potential target for osteosarcoma treatment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available