Journal
VIRUSES-BASEL
Volume 14, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/v14071544
Keywords
classical swine fever (CSF); C-strain; E-rns; competitive ELISA (cELISA); monoclonal antibody; DIVA
Categories
Funding
- National Bio and Agro-Defense Facility Transition Fund
- USDA National Institute of Food and Agriculture [1021491]
- USDA ARS [58-8064-8-011, 58-8064-9-007, 58-3020-9-020, 59-0208-9-222]
- USDA NIFA [2022-67015-36516, 25-6226-0633-002]
- National Pork Board [18-059]
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A novel competitive enzyme-linked immunosorbent assay (cELISA) based on a C-strain E-rns specific monoclonal antibody (mAb 1504) was developed to measure immune responses to C-strain vaccine and make it a DIVA-compatible vaccine.
Classical swine fever can be controlled effectively by vaccination with C-strain vaccine. In this study, we developed a novel competitive enzyme-linked immunosorbent assay (cELISA) based on a C-strain E-rns specific monoclonal antibody (mAb 1504), aiming to serologically measure immune responses to C-strain vaccine in pigs, and finally to make the C-strain become a DIVA-compatible vaccine. The cELISA system was established based on the strategy that mAb 1504 will compete with the C-strain induced antibodies in the pig serum to bind the C-strain E-rns protein. The cELISA was optimized and was further evaluated by testing different categories of pig sera. It can efficiently differentiate C-strain immunized from wild-type CSFV-infected pigs and lacks cross-reaction with other common swine viruses and viruses in genus Pestivirus such as Bovine viral diarrhea virus (BVDV). The C-strain antibody can be tested in pigs 7-14 days post vaccination with this cELISA. The sensitivity and specificity of the established cELISA were 100% (95% confidence interval: 95.60 to 100%) and 100% (95% confidence interval: 98.30 to 100%), respectively. This novel cELISA is a reliable tool for specifically measuring and differentiating immune responses to C-strain vaccine in pigs. By combining with the wild-type CSFV-specific infection tests, it can make the C-strain have DIVA capability.
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