Journal
VIRUSES-BASEL
Volume 14, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/v14071471
Keywords
waterfowl parvovirus; inflammation; ileac microflora; oxidative stress; molecular characterization
Categories
Funding
- Guangdong Basic and Applied Basic Research Foundation [2019B1515210034, 2019A1515012006]
- Key Research and Development Program of Guangdong Province [2020B020222001]
- Construction of Modern Agricultural Science and Technology Innovation Alliance in Guangdong Province [2020KJ128]
- National Natural Science Foundation of China [31902252]
- Special Project of National Modern Agricultural Industrial Technology System [CARS-41]
- National Modern Agricultural Industry Science and Technology Innovation Center in Guangzhou [2018kczx01]
- National Key R&D Program of China [2017YFD0502001]
- Creation of a Triple Chimeric Vaccine (rIBV-ND-H9) Using Avian Infectious Bronchitis Attenuated D90 as a Vector [2017KZDM008]
- Guangzhou Science and Technology Bureau [GZKTP201930]
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This study found that infection with recombinant Muscovy duck parvovirus (rMDPV) can lead to early intestinal barrier dysfunction, inflammation, disturbance of ileal microbiota, and oxidative stress.
Waterfowl parvovirus (WPFs) has multiple effects on the intestinal tract, but the effects of recombinant Muscovy duck parvovirus (rMDPV) have not been elucidated. In this study, 48 one-day-old Muscovy ducklings were divided into an infected group and a control group. Plasma and ileal samples were collected from both groups at 2, 4, 6, and 8 days post-infection (dpi), both six ducklings at a time. Next, we analyzed the genomic sequence of the rMDPV strain. Results showed that the ileal villus structure was destroyed seriously at 4, 6, 8 dpi, and the expression of ZO-1, Occludin, and Claudin-1 decreased at 4, 6 dpi; 4, 6, 8 dpi; and 2, 6 dpi, respectively. Intestinal cytokines IFN-alpha, IL-1 beta and IL-6 increased at 6 dpi; 8 dpi; and 6, 8 dpi, respectively, whereas IL-2 decreased at 6, 8 dpi. The diversity of ileal flora increased significantly at 4 dpi and decreased at 8 dpi. The bacteria Ochrobactrum and Enterococcus increased and decreased at 4, 8 dpi; 2, 4 dpi, respectively. Plasma MDA increased at 2 dpi, SOD, CAT, and T-AOC decreased at 2, 4, 8 dpi; 4, 8 dpi; and 4, 6, 8 dpi, respectively. These results suggest that rMDPV infection led to early intestinal barrier dysfunction, inflammation, ileac microbiota disruption, and oxidative stress.
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