Journal
STRAHLENTHERAPIE UND ONKOLOGIE
Volume 199, Issue 2, Pages 182-191Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00066-022-01978-3
Keywords
Biomarker; Cytogenetics; Gamma rays; Nondisjunction; genetic; Biodosimetry
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By studying the frequency of acrocentric chromosome associations (ACA) in human lymphocytes exposed to gamma radiation, we found that the frequency of ACA increased with radiation dose. We also observed a correlation between ACA frequency and dicentric chromosome (DC) frequency, indicating that ACA may serve as a potential cytogenetic biomarker for radiation biodosimetry.
Purpose The frequency of acrocentric chromosome associations (ACA) was studied to determine the possible dose-response relationship of gamma irradiation in human lymphocytes. Methods Peripheral blood collected from three healthy donors was irradiated with 0, 0.1, 0.25, 0.5, 0.75, 1, 2, 3, 4, and 5 Gy of gamma radiation. Chromosomal preparations were made after 48 h of culture as per standard guidelines. The experiment was repeated three times, with a different donor each time. Results The ACA frequency in irradiated lymphocytes increased with radiation dose. The D-G type of association was most prominent and showed a significant dose-dependent increase in frequency. The dose response of ACA frequency to radiation was found to be linear: ACA frequency = 0.2923 (+/- 0.0276) + 0.1846 (+/- 0.0307) x D (correlation coefficient r = 0.9442). As expected, dicentric chromosome (DC) frequencies followed the linear quadratic fit model, with DC frequency = 0.0015 (+/- 0.0013) + 0.0220 (+/- 0.0059) x D + 0.0215 (+/- 0.0018) x D(boolean AND)2 (correlation coefficient r = 0.9982). A correlation curve was prepared for ACA frequency versus DC frequency, resulting in the regression equation y = 1.130x + 0.4051 (R-2 = 0.7408; p = 0.0014). Conclusion Our results showed an increase in ACA frequency in irradiated lymphocytes with an increase in radiation dose; thus, ACA may serve as a candidate cytogenetic biomarker for radiation biodosimetry.
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