Journal
STEM CELL RESEARCH
Volume 63, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scr.2022.102870
Keywords
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Funding
- Slovak Research and Development Agency grant [APVV-17-0037]
- European Regional Development Fund [67985904]
- RVO [APVV-17-0037]
- [313011AFG5]
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In this study, a new iPS cell line was generated for modeling the sporadic form of ALS. The cell line was derived from skin fibroblasts obtained through reprogramming and characterized through various analyses, demonstrating its normal karyotype and ability to differentiate into cells from three germ layers.
We present here a new iPS cell line for modeling sporadic form of ALS. Cell line was generated by reprogramming skin fibroblasts isolated with explant culture technology from skin biopsy, donated by ALS patient. For reprogramming, polycistronic self-replicating RNA vector was used and derived iPS cells were charac-terized by immunocytochemistry and FACS (pluripotent factors expression), karyotyping, STR fingerprinting analysis and in vitro differentiation assay. New cell line showed normal (46, XY) karyotype and differentiated in vitro into cells from three germ layers. STR analysis proved the origin and originality of the cell line.
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