A novel hydrogen peroxide fluorescent probe for bioimaging detection and enables multiple redox cycles

In this subject, a novel hydrogen peroxide (H2O2) fluorescent probe (MNG) was designed and developed using naphthalimide derivatives and selenomorpholine. In PBS buffer (10 mM, pH = 7.4, 1 %DMSO), the selenomorpholine on the probe is capable of qualitatively and quantitatively detecting (H2O2) at a small amount under a detection limit of 61 nM. The probe follows a mechanism that Se (II) in selenomorpholine is transformed to Se (IV), thus changing the spectra of the probe MNG. It is noteworthy that MNG can continuously make a cyclic response to H(2)O(2 )and glutathione (GSH), so it can potentially achieve redox process imaging in vivo. Moreover, this subject verified the redox process of the probe's continuous redox response in the Gaussian 09 programme through simulation calculation and mass spectrometry. The probe exhibits high biocompatibility. Moreover, it can detect H2O2 in MCF-7 cells and Argentine Bloodfin. (C) 2022 Elsevier B.V. All rights reserved.

Automated summary

In this study, a novel fluorescent probe for hydrogen peroxide (H2O2) detection was designed and developed. The probe showed high biocompatibility and the ability to achieve redox process imaging in vivo.