Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 276, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2022.121230
Keywords
Fluorescent probe; Cysteine; Large Stokes shift; Biological thiols
Categories
Funding
- National Natural Science Foundation of China [21672083, 21877048, 22077048]
- Guangxi Natural Science Foundation [2021GXNSFDA075003, AD21220061]
- Guangxi University [A3040051003]
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Small molecule biological thiols play crucial roles in maintaining cellular activities and abnormal levels are associated with diseases. A new fluorescent probe Ty-Cys1 was designed in this study for effective monitoring of Cys changes.
The small molecule biological thiols, such as Cysteine (Cys), homocysteine (Hcy), and glutathione (GSH), play crucial roles in maintaining various cellular vital activities. In the organism, abnormal levels of small-molecule biological thiols have been associated with a variety of diseases. Therefore, quantitative determination of biological thiols, especially Cys, is significant for understanding their functions in various biological processes. Thus, in this work we designed a new fluorescent probe Ty-Cys1 with a large Stokes shift of 207 nm to monitor Cysteine. The maximum absorption wavelength of Ty-Cys1 was 418 nm, and the maximum emission wavelength was 625 nm. Significantly, the novel probe Ty-Cys1 was effectively in detecting of Cys changes in living cells, zebrafishes, and living mice. (c) 2022 Elsevier B.V. All rights reserved.
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