Journal
SLOVENIAN VETERINARY RESEARCH
Volume 59, Issue 2, Pages 89-98Publisher
UNIV LJUBLJANA, VETERINARY FACULTY
DOI: 10.26873/SVR-1316-2022
Keywords
SNP; haplotype; leptin receptor; selection; Japanese quail
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Funding
- Scientific Research Projects Coordination Unit of Akdeniz University [2012.01.0104.002]
- Scientific and Technological Research Council of Turkey [:114O047]
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This study investigated the effects of selection on the SNPs in the coding sequence of the LEPR gene and their associations with meat yield traits in Japanese quail lines. The results showed that selection caused significant changes in the LEPR gene and had an impact on economically important traits.
This study was carried out to investigate the effects of selection on the single nucleotide polymorphisms (SNPs) in coding sequence of leptin receptor (LEPR) gene and possible associations between SNPs' and some meat yield traits of Japanese quail lines. Fifteen generations divergently selected two lines (HBW and LBW) for 5-weeks of age body weight and a control were used as materials for this study. A348-bp part of the LEPR coding region (18th exon) were sequenced in a total of 113 individuals from the three quail lines and shown that the fragments contained four SNPs loci (T490C, C528T, G537A, T571C) and five haplotypes (TTGT, CTGT, TCGT, TCAT, TCAC). T490C replacement caused the missense mutation of phenylalanine to convert to leucine (Phe>Leu). However, other SNPs were synonymous and there were no changes in transcripts. It was determined that the quails with higher phenotypic values were in the TT genotype at the T390C locus. Statistical analyses showed that there were significant differences among the quail lines, SNP alleles and haplotypes in terms of interested phenotypic traits (P<0.05), and also SNP and haplotype distributions changed depending on quail lines (P<0.001). When all results were evaluated together, it was concluded that the fifteen generations of selection caused significant changes in the LEPR gene in terms of economically important traits in Japanese quail lines (C. coturnix japonica).
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