A highly sensitive optical fiber based near-infrared laser induced fluorescence detector (LIF) for parathyroid gland detection

A highly sensitive optical fiber based laser-induced fluorescence (LIF) detector was developed for identification of in-vitro parathyroid gland (PG). An optical fiber probe with included angle of 20 degrees was designed to reduce the blind area and collection of reflected light. Compared with collinear bundles probe, the sensitivity was increased by 53.4% (converted to equivalent collection area), and the short-term fluctuation and long-term drift were reduced by 61.1% and 58.3%, respectively. Besides, by adding the designed dichroic mirror module to the emission optical path, the baseline and noise were further reduced by 96.7% and 92.1%, respectively, and the signal-to-noise ratio (SNR) was improved by about 9 times. The limit of detection (LOD) for CF790 dye was 5.1 x 10(-14) mol/L (SNR=3, peak-to-peak noise) under evaluation of standard cuvette, which was hundreds times lower than other optical fiber based LIFs. Then, the detector was applied to detect in-vitro diseased PG samples, with diagnostic accuracy of 83.3%, which met the demand of clinical PG detection.

Automated summary

A highly sensitive optical fiber-based laser-induced fluorescence (LIF) detector was developed for in-vitro parathyroid gland (PG) identification. By designing a special optical fiber probe and adding a dichroic mirror module, the sensitivity was increased and the noise was reduced, leading to more accurate detection results.