Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 365, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.131871
Keywords
Hygiene indicator bacteria; Food contamination; CRSPR; Cas; Point-of-care; Portable detector; Direct-PCR
Funding
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Agro and Livestock Products Safety Flow Management Technology Development Program - Ministry of Agriculture, Food and Rural Affairs (MAFRA) [IPET319112-2]
- National Research Foundation of Korea (NRF) - Korea Government (Ministry of Science and ICT) [NRF-2020R1C1C1012275]
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The study developed a point-of-care platform for the rapid and specific detection of hygiene indicator bacteria, including E. coli, coliform, and total bacteria, using a dual recognition process by PCR and CRISPR/Cas. The system achieved a low detection sensitivity and demonstrated its utility for food hygiene monitoring through spiking tests in real samples. It represents a key platform for sensitive and specific detection of hygiene indicator bacteria in resource- or facility-limited areas.
A rapid and sensitive diagnostic system is essential for the on-site monitoring of sanitary conditions in the food industry. In this study, we developed a point-of-care platform for the detection of hygiene indicator bacteria, including E. coli, coliform, and total bacteria, using a portable fluorescence detector. Through the dual recognition process by PCR and CRISPR/Cas, our system enables the simultaneous detection of bacteria in three different groups with the high specificity. The optimal reaction conditions obtained by the systematic investigation achieved a detection sensitivity as low as 10-1 cells/mu L (1 cell/reaction). Furthermore, spiking tests in real samples were successfully carried out, which demonstrated the utility of our portable system for food hygiene monitoring. The proposed system represents a key platform for the sensitive and specific detection of hygiene indicator bacteria in resource- or facility-limited areas.
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