Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 362, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.131830
Keywords
Paper-based platform; Tetrahedral DNA nanostructure; Colorimetric; Mercury
Funding
- National Natural ScienceFoundat i o n of China [61801274,21705139,41776110, 22106179]
- Natural Science Foundation of Shandong Province [ZR2020KB022]
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A paper-based colorimetric sensor functionalized with tetrahedral DNA nanostructure (TDN) was developed for ultrasensitive analysis of mercury ion (Hg2+). The sensor exhibited high capture efficiency and low detection limit, and was successfully applied in real water samples.
A novel tetrahedral DNA nanostructure (TDN) functionalized paper-based colorimetric sensor was developed for ultrasensitive analysis of mercury ion (Hg2+). On the paper-based platform, TDN was used as scaffold to anchor aptamer to improve the capture efficiency owing to the controllably specific orientation, low steric hindrance effect and well-defined spacing. In the absence of Hg2+, biotinylated aptamer would bind with streptavidinlabeled HRP. Whereas, in the presence of Hg2+, aptamer would preferentially bind to Hg2+ owing to the higher binding constant of T-Hg-T than that of T-A, which consequently leading to the biotinylated aptamer releasing from TDN. After introducing TMB-H2O2 solution, strong variation of color signal could be observed. Therefore, the quantitative analysis of Hg2+ was carried out by using smartphone to monitor the color intensity on paper chip. The proposed strategy exhibits good linearity over a wide range of 10(-13)-10(-7) M with a low detection limit of 30 fM. And this method had been successfully applied in real water samples. Our strategy provided a versatile, low-cost and user-friendly paper-based monitoring platform for rapid and ultrasensitive analysis of heavy metal ions and thus had potential applications in food safety and public health.
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