Wastewater-derived biohydrogen: Critical analysis of related enzymatic processes at the research and large scales

Organic-rich wastewater is a feasible feedstock for biohydrogen production. Numerous review on the performance of microorganisms and the diversity of their communities during a biohydrogen process were published. However, there is still no in-depth overview of enzymes for biohydrogen production from wastewater and their scale-up applications. This review aims at providing an insightful exploration of critical discussion in terms of: (i) the roles and applications of enzymes in wastewater-based biohydrogen fermentation; (ii) systematical introduction to the enzymatic processes of photo fermentation and dark fermentation; (iii) parameters that affect enzymatic performances and measures for enzyme activity/ability enhancement; (iv) biohydrogen production bioreactors; as well as (v) enzymatic biohydrogen production systems and their larger scales application. Furthermore, to assess the best applications of enzymes in biohydrogen production from wastewater, existing problems and feasible future studies on the development of low-cost enzyme production methods and immobilized enzymes, the construction of multiple enzyme cooperation systems, the study of biohydrogen production mechanisms, more effective bioreactor exploration, larger scales enzymatic biohydrogen production, and the enhancement of enzyme activity or ability are also addressed.

Automated summary

This review provides an insightful exploration of enzymes in wastewater-based biohydrogen fermentation, including their roles, enzymatic processes, parameters affecting enzymatic performances, bioreactors, and larger scale applications. It also addresses existing problems and feasible future studies on low-cost enzyme production methods, the construction of multiple enzyme cooperation systems, biohydrogen production mechanisms, effective bioreactor exploration, larger scale enzymatic biohydrogen production, and the enhancement of enzyme activity or ability.