Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 829, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2022.154691
Keywords
Synechocystis sp; Bicarbonate; Polyhydroxyalkanoates (PHA); Glycogen; Metabolism
Categories
Funding
- Spanish Ministry of Science, Innovation and Universities (MCIU)
- Research National Agency (AEI)
- European Regional Development Fund (FEDER) [AL4BIO, RTI2018-099495-B-C21]
- Spanish Ministry of Education, Culture and Sport [FPU18/04941]
- Spanish Ministry of Industry and Economy [IJC2019-042069-I]
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This study evaluated the effect of different inorganic carbon concentrations on a Synechocystis sp. strain isolated from wastewater, and found that 2 gC/L of bicarbonate stimulated cell growth and PHB production. Gene expression analysis revealed a close relationship between PHB synthesis and glycogen catabolism.
Cyanobacteria are capable of transforming CO2 into polyhydroxybutyrate (PHB). In this study, different inorganic carbon concentrations (0-2 gC L-1) were evaluated for a Synechocystis sp. strain isolated from wastewater. Quantitative RT-qPCR was also performed to decipher the links between inorganic carbon and PHB and glycogen metabolism. 2 gC L-1 of bicarbonate stimulated cell growth, nutrients consumption and production of PHB. Using this concentration, a 14%dcw of PHB and an average productivity of 2.45 mgPHB L-1 d-1 were obtained. Gene expression analysis revelated that these conditions caused the overexpression of genes related to glycogen and PHB synthesis. Moreover, a positive correlation between the genes codifying for the glycogen phosphorylase, the acetyl-CoA reductase and the poly(3-hydroxyalkanoate) polymerase was found, meaning that PHB synthesis and glycogen catabolism are strongly related. These results provide an exhaustive evaluation of the effect of carbon on the PHB production and cyanobacterial metabolism.
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