Nano-bio interfacial interactions determined the contact toxicity of nTiO2 to nematodes in various soils

The biological effect of soilborne nanoparticles (NPs) is a manifestation of soil-NMs-bio interactions. Soil factors are known to restructure NPs surfaces and thus influence the nanotoxicity. However, the mechanisms by which environmental factors affecting nano-bio interactions to aggravate or alleviate nanotoxicities are poorly understood. Herein, we compared the toxicity of TiO2 NPs (nTiO(2)) in five soils using the model nematode (Caenorhabditis elegans), and investigated the variation of nano-bio interactions under different conditions. A correlation analysis showed that pH and dissolved organic matter (DOM) were dominant regulators of nTiO(2) toxicity. At the nano-bio interface, low pH (5.0) led to nTiO(2) adhesion to micron-sized furrows and aggravated dermal wrinkling, while humid acid (HA) alleviated these impacts. Mechanically, low pH increased nTiO(2) adhesion through enhanced electrostatic attraction and subsequent stimulation of mucin and collagen synthesis, resulting in a positive feed cycle of pH-dependent contact nanotoxicity. HA not only prevented nTiO(2) adhesion onto the epidermis due to its negative charge, but also relieved the overstimulation of stress response pathways, thereby alleviating nanotoxicity. These findings broaden our knowledge of how NPs induce contact toxicity in soil invertebrates through specific biointerfacial interactions, and highlight the important role of DOM in alleviating the combined hazards of NPs and soil acidification.

Automated summary

The biological effect of soilborne nanoparticles is a result of the interactions between soil, nanoparticles, and organisms. Soil factors can change the surface properties of nanoparticles and therefore affect their toxicity. However, the mechanisms by which environmental factors influence nano-bio interactions to exacerbate or alleviate nanotoxicities are not well understood. This study investigated the toxicity of TiO2 nanoparticles in different soils and found that pH and dissolved organic matter were the main regulators of nanoparticle toxicity. Low pH increased nanoparticle adhesion and dermal wrinkling, while humid acid alleviated these effects. These findings enhance our understanding of how nanoparticles induce contact toxicity in soil invertebrates through specific biointerfacial interactions and emphasize the important role of dissolved organic matter in mitigating the combined hazards of nanoparticles and soil acidification.