Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 831, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2022.154857
Keywords
E. coli; Portable biosensor; Aptamer; CdTe MPA QDs; SPIONs
Categories
Funding
- DFRL (DRDO), Government of India [1634/PC]
- UGC, New Delhi, India
- DBT [BT/PR27444/BRB/10/1645/2018]
- ICMR, Government of India [35/2/2020-Nano/BMS]
- DHR, Government of India [YSS/2020/000047/PRCYSS]
Ask authors/readers for more resources
In this study, a prototype biosensor using magnetic nanoparticles and fluorescent probes was developed for rapid and accurate detection of Escherichia coli in water and food samples.
The contamination of food and potable water with microorganisms may cause food-borne and water-borne diseases. The common contaminants include Escherichia coli (E. coli), Salmonella sp. etc. The conventional methods for monitoring the water quality for the presence of bacterial contaminants are time-consuming, expensive, and not suitable for rapid on-spot detection in field conditions. In the current study, super paramagnetic iron oxide nanoparticles (SPIONs) were synthesized and conjugated with E. coli specific Aptamer I to detect E. coli cells qualitatively as well as quantitatively. The sludge consisting of E. coli- SPION complex was separated via magnetic separation. The presence of E. coli cells was confirmed with the help of standard techniques and confocal laser scanning microscopy (CLSM) employing Aptamer II conjugated CdTe-MPA quantum dots (QDs). Finally, an ATmega 328P prototype biosensor based on Aptamer II conjugated CdTe MPA QDs exhibited quantitative and qualitative abilities to detect E.coli. This prototype biosensor can even detect low bacterial counts (up to 1x10(2) cfu) with the help of a photodiode and plano-convex lens. Further, the prototype biosensor made up of ultraviolet light-emitting diode (UV LED), liquid crystal display (LCD) and ATmega328Pmicrocontroller offers on-spot detection of E.coli in water samples with high resolution and sensitivity. Similarly, this in-house developed prototype biosensor can also be utilized to detect bacterial contamination in food samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available