Distribution, influence factors, and biotoxicity of environmentally persistent free radical in soil at a typical coking plant

Environmentally persistent free radicals (EPFRs) are emerging pollutants in contaminated soils and have attracted significant attention. Chinese coke production making a great contribution to the globe is increasingly identified as the non-ignorable source of EPFRs. However, the distribution level, influence factors, and biotoxicity of EPFRs at coking sites remain poorly understood. Herein, a typical coking plant in Tangshan, China, featuring two functional regions (the reconstructed project (RP) and elimination engineering (EE)) was used to study the existence of EPFRs. The spin density of the EPFRs in coking soils was 3.20 x 1020-3.11 x 1021 spins/g with g-factor values of 2.0020-2.0036. The EPFRs presented higher concentrations and g-factor values in RP region than in EE region, and a mixture of carbon-centered radicals and carbon-centered radicals with adjacent oxygen atoms as well as carbon centered radical was ascertained in the former and the latter, respectively. Correlation analysis and FT-ICR-MS results indicated that polycyclic aromatic hydrocarbons (PAH) together with other unsaturated hydrocarbons and condensed aromatic contaminants, might contribute to the EPFRs formation in the soils of RP region, whereas PAHs were the main source of EPFRs in EE region. Soil components were determined to investigate the influence factors in EPFRs formation. Cu and Fe2O3 were recognized as the markedly positive influence factors, while TOC had a negative impact on EPFR formation. Visible light irradiation can induce the transformation and generation of EPFRs. As representative contaminants, both toluene and 2-chlorophenol can create EPFRs in coking soil under visible light irradiation. The potential biotoxicity tests of Photobacterium phosphoreum T3 spp. showed that EPFRs from the soils diminished bacterial

Automated summary

Environmentally persistent free radicals (EPFRs) are emerging pollutants in contaminated soils, with Chinese coke production identified as a significant source. A study on a typical coking plant in Tangshan, China revealed higher concentrations of EPFRs in the reconstructed project (RP) region compared to the elimination engineering (EE) region, with polycyclic aromatic hydrocarbons (PAH) identified as the main source in EE region. Copper (Cu) and iron oxide (Fe2O3) were found to be positive influence factors in EPFRs formation, while total organic carbon (TOC) had a negative impact. Visible light irradiation was shown to induce the transformation and generation of EPFRs.