Improving the efficiency of prime editing with epegRNAs and high-temperature treatment in rice

Article Biotechnology & Applied Microbiology

Engineered pegRNAs improve prime editing efficiency

James W. Nelson et al.

Summary: Prime editing technology enhances editing efficiency by optimizing the structure of pegRNAs, preventing degradation of the 3' region from affecting system activity, without increasing the risk of off-target editing.

NATURE BIOTECHNOLOGY (2022)

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Review Biology

Rice functional genomics: decades' efforts and roads ahead

Rongzhi Chen et al.

Summary: Rice, as one of the most important crops in the world, has seen significant progress in understanding molecular mechanisms and regulatory networks in various rice traits over the past few decades. Advanced technologies such as new genomics methods, high-throughput phenotyping platforms, precise genome-editing tools, environmental microbiome optimization, and synthetic methods will further enhance our understanding of unresolved molecular biology questions in rice and facilitate their integration into agricultural applications.

SCIENCE CHINA-LIFE SCIENCES (2022)

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Article Biotechnology & Applied Microbiology

An engineered prime editor with enhanced editing efficiency in plants

Yuan Zong et al.

Summary: In this study, we have improved the editing efficiency of prime editing by engineering the structure of the prime editor and combining it with specific guide RNAs. The optimized prime editor showed significantly increased editing efficiency in plant cells, and when combined with engineered guide RNAs, it resulted in the generation of herbicide-tolerant rice plants.

NATURE BIOTECHNOLOGY (2022)

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Article Plant Sciences

A design optimized prime editor with expanded scope and capability in plants

Wen Xu et al.

Summary: This study demonstrated that using N-terminal reverse transcriptase-Cas9 nickase fusion and introducing multiple-nucleotide substitutions in the reverse transcriptase template can significantly enhance the efficiency of prime editing in plants and human cells. The optimized approach achieved higher editing frequencies compared to the original editor, Prime Editor 3, showing the potential to make previously non-editable target sites editable and expand the capabilities of prime editing in the future.

NATURE PLANTS (2022)

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Letter Biotechnology & Applied Microbiology