Manipulation of coacervate droplets with an electric field

Many biopolymers are highly charged, and as in the case of many polymer mixtures, they tend to phase separate as a natural consequence of chain connectivity and an associated relatively low entropy of polymer mixing. Recently, it has become appreciated that the phase-separated structures formed by such polyelectrolyte blends, called complex coacervates, underlie numerous biological structures and processes essential to living systems, and there has been intense interest in understanding the unique physical features of this type of phase-separation process. In the present work, we are particularly concerned with the field responsiveness of stabilized coacervate droplets formed after the phase separation of polyelectrolyte blend solution and then exposed to deionized water, making the droplet interfacial layer acquire a viscoelastic character that strongly stabilizes it against coalescence. We show that we can precisely control the positions of individual droplets and arrays of them with relatively low-voltage electric fields (on the order of 10 V/cm) and that the imposition of an oscillatory field gives rise to chain formation with coarsening of these chains into long fibers. Such a phase-separation-like process is generally observed in electrorheological fluids of solid colloidal particles subjected to much larger field strengths. The key to these coacervates' electrorheological properties is the altered interfacial viscoelastic properties when the droplets are introduced into deionized water and the associated high polarizability of the droplets, similar to the properties of many living cells. Since many different molecular payloads can be incorporated into these stable droplets, we anticipate many applications.

Automated summary

Many biopolymers undergo phase separation due to chain connectivity and low entropy of mixing. This phase separation process, called complex coacervation, plays a vital role in biological structures and processes. In this study, we investigate the field responsiveness of stabilized coacervate droplets after phase separation and exposure to deionized water. We find that by applying low-voltage electric fields, we can precisely control the position of individual droplets and induce chain formation. This phase separation-like process resembles the behavior of electrorheological fluids and is attributed to altered interfacial viscoelastic properties and high polarizability of the droplets.