4.8 Article

Viral evasion of PKR restriction by reprogramming cellular stress granules

Publisher

NATL ACAD SCIENCES
DOI: 10.1073/pnas.2201169119

Keywords

PKR; stress granules; PRRSV; inflammation; nsp1 beta

Funding

  1. National Natural Science Foundation of China [32025035, 31772759]
  2. China Agriculture Research System of Ministry of Finance and Ministry of Agriculture and Rural Affairs [CARS-35]
  3. China Postdoctoral Science Foundation [2021M703527]

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This study reveals that porcine reproductive and respiratory syndrome virus (PRRSV) utilizes its replicase protein nsp1 beta to suppress the activation of host protein kinase R (PKR) by repurposing stress granules (SGs), resulting in the inhibition of host inflammatory responses.
Protein kinase R (PKR) is a critical host restriction factor against invading viral pathogens. However, this molecule is inactivated in the cells infected with porcine reproductive and respiratory syndrome virus (PRRSV), an economically devastating pathogen to the world swine industry. Here, we report that this event is to suppress cellular inflammation and is mediated by the viral replicase protein nsp1 beta. We show that nsp1 beta is a stress-responsive protein, enters virus-induced stress granules (SGs) during infection, and repurposes SGs into a proviral platform, where it co-opts the SG core component G3BP1 to interact with PKR in a regulated manner. RNA interference silencing of G3BP1 or mutation of specific nsp1 beta residues (VS19GG) can abolish the antagonization of PKR activation. The viral mutant carrying the corresponding mutations induces elevated level of PKR phosphorylation and pronounced production of inflammatory cytokines (e.g., tumor necrosis factora, interleukin [IL]-6, and IL-8), whereas small-interfering RNA knockdown of PKR or treatment with C16, a PKR inhibitor, blocks this effect. Thus, PRRSV has evolved a unique strategy to evade PKR restriction to suppress host inflammatory responses.

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