Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 119, Issue 26, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2119101119
Keywords
cryo-EM tomography; electron microscopy; deconvolution; chromosome structure
Categories
Funding
- University of California, San Diego School of Medicine Microscopy Core [P30 NS047101]
- NIH [P41-GM103311, I082850, R0AI00880, A102853]
- NSF BSF [2019280]
- Israel Science Foundation [1696/18]
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Cryoelectron tomography of the cell nucleus using scanning transmission electron microscopy and deconvolution processing technology has revealed a large-scale chromosome structure. This study further investigates and analyzes these structures, proposing a unified architecture for interphase chromosomes.
Cryoelectron tomography of the cell nucleus using scanning transmission electron microscopy and deconvolution processing technology has highlighted a large-scale, 100 to 300-nm interphase chromosome structure, which is present throughout the nucleus. This study further documents and analyzes these chromosome structures. The paper is divided into four parts: 1) evidence (preliminary) for a unified interphase chromosome structure; 2) a proposed unified interphase chromosome architecture; 3) organization as chromosome territories (e.g., fitting the 46 human chromosomes into a 10-mu m-diameter nucleus); and 4) structure unification into a polytene chromosome architecture and lampbrush chromosomes. Finally, the paper concludes with a living light microscopy cell study showing that the G1 nucleus contains very similar structures throughout. The main finding is that this chromosome structure appears to coil the 11-nm nucleosome fiber into a defined hollow structure, analogous to a Slinky helical spring [https:// en.wikipedia.org/wiki/Slinky; motif used in Bowerman et al., eLife 10, e65587 (2021)]. This Slinky architecture can be used to build chromosome territories, extended to the polytene chromosome structure, as well as to the structure of lampbrush chromosomes.
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