Journal
POULTRY SCIENCE
Volume 101, Issue 10, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.psj.2022.102084
Keywords
cholamine; egg production; intestinal morphology; lipid metabolism; microbiota
Categories
Funding
- Jiangsu Province Major Agricultural New Varieties Creation Project [PZCZ201731]
- Joint International Research Laboratory of Agriculture and Agri -Product Safety of the Ministry of Education of China
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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The study found that supplementary cholamine may lower egg production by promoting hepatic lipid deposition, reducing abundances of beneficial intestinal bacteria, and decreasing microfloral biodiversity in laying hens.
The effects of cholamine, a raw material for synthesis of some active lipids, are unknown in poul-try. To address this, 180 52-wk-old Hyline laying hens were randomly divided into 3 groups (20 replicates per group with three hens per replicate). The control group and the treatment groups (treatment 1 and 2) were fed basal diet and the diet supplemented with 500 or 1,000 mg of cholamine per kilogram of the diet for 35 d, respectively. The data showed that supplementary chol-amine significantly lowered egg production, daily feed intake, serum high-density lipoprotein cholesterol level, liver index, and the percentages of C15:0 and C20:0 in fatty acid composition of liver, significantly elevated hepatic triglyceride content, the ratio of villus height to crypt depth (P < 0.05), and the percentage of C18:2n-6 and the ratio of n-6 to n-3 polyunsaturated fatty acids in liver fat (P < 0.10). Moreover, supplementary cholamine altered the relative abundance of some intes-tinal bacteria with a decrease in the alpha biodiversity (P < 0.10). Additionally, transcriptome analysis on the livers of the treatment vs. the control groups identified 1,151 up-and 914 down-regulated differentially expressed genes (DEGs), and pathway analysis revealed that the suppressed Notch signaling pathway and the enhanced Oxidative phosphorylation pathway were enriched with DEGs. Particularly, fat absorption, transport and oxidative phosphorylation-related DEGs (e.g., FABP1, APOA4, and PCK1) were significantly induced, but fatty acid synthesis, and lipid package and secretion-related DEGs (e.g., FASN, SCD, and MTTP) were not. In conclusion, supplementary cholamine may lower egg production by promoting hepatic lipid deposi-tion and reducing abundances of beneficial intestinal bacteria and microfloral biodiversity in laying hens.
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