A bacterial dual positive and negative selection system for dCas9 activity

The engineering of switchable or activatable dCas9 proteins would benefit from a single system for both positive and negative selection of dCas9 activity. Most systems that are used to interrogate dCas9 libraries use a fluorescent protein screen or an antibiotic selection for active dCas9 variants. To avoid some of the limitations of these systems, we have developed a single system capable of selecting for either active or inactive dCas9 variants. E. coli expressing active dCas9 variants are isolated in the positive selection system through growth in the presence of ampicillin. The negative selection can isolate cells lacking dCas9 activity through two separate mechanisms: growth in M9 minimal media or growth in media containing streptomycin. This system is capable of enriching for rare dCas9 variants up to 9,000-fold and possesses potential utility in directed evolution experiments to create switchable dCas9 proteins.

Automated summary

A single system has been developed for selecting both active and inactive dCas9 variants. Active variants are selected through growth in the presence of ampicillin, while cells lacking dCas9 activity are isolated through growth in M9 minimal media or media containing streptomycin. This system can enrich for rare dCas9 variants up to 9,000-fold and has potential utility in directed evolution experiments.