4.7 Article

Production and characterization of a novel interspecific somatic hybrid combining drought tolerance and high quality of sweet potato and Ipomoea triloba L.

Journal

PLANT CELL REPORTS
Volume 41, Issue 11, Pages 2159-2171

Publisher

SPRINGER
DOI: 10.1007/s00299-022-02912-8

Keywords

Drought tolerance; Genetic and epigenetic variations; Ipomoea triloba; Quality; Somatic hybrid; Sweet potato

Categories

Funding

  1. National Key R&D Program of China [2019YFD1001302/2019YFD1001300]
  2. earmarked fund for CARS-10-Sweet potato

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A novel interspecific somatic hybrid, XT1, was obtained through protoplast fusion between sweet potato cv. Xushu 18 and its wild relative I. triloba. This somatic hybrid exhibited both drought tolerance and high quality of storage roots. Transcriptome and DNA analysis showed the up-regulation of drought stress-responsive genes and genomic structural reconstructions in the hybrid. This study highlights the significance of somatic hybridization in the genetic improvement of sweet potato and provides valuable resources for further investigation of storage root formation.
Key message A novel interspecific somatic hybrid combining drought tolerance and high quality of sweet potato andIpomoea triloba L. was obtained and its genetic and epigenetic variations were studied. Somatic hybridization can be used to overcome the cross-incompatibility between sweet potato (Ipomoea batatas (L.) Lam.) and its wild relatives and transfer useful and desirable genes from wild relatives to cultivated plants. However, most of the interspecific somatic hybrids obtained to date cannot produce storage roots and do not exhibit agronomic characters. In the present study, a novel interspecific somatic hybrid, named XT1, was obtained through protoplast fusion between sweet potato cv. Xushu 18 and its wild relative I. triloba. This somatic hybrid produced storage roots and exhibited significantly higher drought tolerance and quality compared with its cultivated parent Xushu 18. Transcriptome and real-time quantitative PCR (qRT-PCR) analyses revealed that the well-known drought stress-responsive genes in XT1 and I. triloba were significantly up-regulated under drought stress. The genomic structural reconstructions between the two genomes of the fusion parents in XT1 were confirmed using genomic in situ hybridization (GISH) and specific nuclear and cytoplasmic DNA markers. The DNA methylation variations were characterized by methylation-sensitive amplified polymorphism (MSAP). This study not only reveals the significance of somatic hybridization in the genetic improvement of sweet potato but also provides valuable materials and knowledge for further investigating the mechanism of storage root formation in sweet potato.

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