Journal
PLANT BIOTECHNOLOGY JOURNAL
Volume 20, Issue 9, Pages 1786-1806Publisher
WILEY
DOI: 10.1111/pbi.13864
Keywords
synthetic gene circuits; rice; cell-type-specific gene expression; dTALE-STAP
Funding
- National Collaborative Research Infrastructure Strategy of the Australian Government
- BMBF from the German Federal Ministry for Education and Research [031B0548]
- BBSRC sLOLA grant [BB/P003117/1]
- Royal Society University Research Fellow
- Advanced Plant Biotechnology Center from the Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan
- Academia Sinica
- Bill & Melinda Gates Foundation [OPP1129902, INV-002970]
- BBSRC [BB/P003117/1] Funding Source: UKRI
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In biological research, controlling transgene expression spatially and temporally is crucial. However, the limited availability of specific tissue-specific and/or time-specific promoter sequences often hinders the co-expression of multiple transgenes in precisely controlled developmental contexts. In this study, researchers developed a system for rice that utilizes synthetic designer transcription activator-like effectors (dTALEs) and synthetic TALE-activated promoters (STAPs) to allow expression of multiple transgenes from different promoters. The system demonstrated successful activation of reporter gene expression in stable transgenic rice lines, with transgene transcript levels dependent on the sequence identities of dTALEs and STAPs. The system has the potential to fine-tune the expression of multiple transgenes and introduce different synthetic circuits into distinct developmental contexts.
In biological discovery and engineering research, there is a need to spatially and/or temporally regulate transgene expression. However, the limited availability of promoter sequences that are uniquely active in specific tissue-types and/or at specific times often precludes co-expression of multiple transgenes in precisely controlled developmental contexts. Here, we developed a system for use in rice that comprises synthetic designer transcription activator-like effectors (dTALEs) and cognate synthetic TALE-activated promoters (STAPs). The system allows multiple transgenes to be expressed from different STAPs, with the spatial and temporal context determined by a single promoter that drives expression of the dTALE. We show that two different systems-dTALE1-STAP1 and dTALE2-STAP2-can activate STAP-driven reporter gene expression in stable transgenic rice lines, with transgene transcript levels dependent on both dTALE and STAP sequence identities. The relative strength of individual STAP sequences is consistent between dTALE1 and dTALE2 systems but differs between cell-types, requiring empirical evaluation in each case. dTALE expression leads to off-target activation of endogenous genes but the number of genes affected is substantially less than the number impacted by the somaclonal variation that occurs during the regeneration of transformed plants. With the potential to design fully orthogonal dTALEs for any genome of interest, the dTALE-STAP system thus provides a powerful approach to fine-tune the expression of multiple transgenes, and to simultaneously introduce different synthetic circuits into distinct developmental contexts.
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